CAS:64232-83-3L189

L189 is a human DNA ligase inhibitor, inhibits hLigI/III/IV with IC50 of 5/9/5 μM.

规格	价格	促销价格	库存

18817579266

立即询价

咨询

18817579266

立即询价

实验数据

技术信息

CAS号

64232-83-3

储存条件

分子式

C11H10N4OS

运输

蓝冰

分子量

246.29

别名

溶解度

动物实验配方

生物活性

描述Multiple DNA ligation events are required to join the Okazaki fragments generated during lagging strand DNA synthesis. In eukaryotes, this is primarily carried out by members of the DNA ligase family^[2]. L189 is a novel and competitive inhibitor of human DNA ligase family with IC₅₀ values of 5, 9 and 5 μM for hLigI (DNA ligases I), hLigIII, and hLigIV, respectively. L189 inhibited step 2 of the ligation reaction catalyzed by all three human DNA ligases by at least 90%. L189 reduced the proliferation and/or viability of four human cell lines, including a normal breast epithelial cell line MCF10A and the cancer cell lines MCF7, HeLa, and HCT116 established from breast, cervical, and colon cancers, respectively, in a concentration-dependent manner. L189 also enhanced the killing of cancer cell lines but not the normal cell line by MMS. Furthermore, L189 markedly increased the killing of HCT116 colon cancer cells and the other cancer cell lines by ionizing radiation^[3].
作用机制L189 binds to the DNA-binding pocket within the DBD (DNA binding domain) of hLigI^[3].