生物活性

• 描述The herpesviruses comprise a large family of viruses infecting most vertebrates. The virus particle is about 100 nm in diameter and consists of an icosahedral nucleocapsid surrounded by an outer membrane. A critical defining property of the herpesviruses is their ability to form a latent infection as part of their life cycle. Adefovir has been shown to have good antiviral activity against several viruses, including HIV and HBV, and herpesviruses, such as HSV (herpes simplex virus), EBV (Epstein–Barr virus), VZV (varicella zoster virus), and CMV (cytomegalovirus). Adefovir has an IC_{50 of 0.7 μM against HBV in the HepG2.2.15 cell line^[3]. The effect of adefovir on viral CCC DNA (covalently closed circular DNA) synthesis was examined with primary cultures of DHBV (duck HBV)-infected fetal hepatocytes. Adefovir was administered for six consecutive days starting one day before or four days after DHBV inoculation. Dose-dependent inhibition of both virion release in culture supernatants and synthesis of intracellular viral DNA was observed. Preventive treatment of experimentally infected ducklings with adefovir revealed that it efficiently suppressed viremia and intrahepatic DNA^[4]. The steady-state volume of distribution of adefovir in patients was found to be about 0.4 L/kg^[3].}