生物活性

• 描述Acetylcholine receptor (AChR) including muscarinic AChRs and nicotinic AChRs can bind acetylcholine and transmit its signal. Acetylcholine chloride is a potent AChR inhibitor with different IC50 values in different cell types. In sweat gland epithelial cells, experiment groups A1–6 were cultured with 2 mM calcium and stimulated with 0.5 μM acetylcholine chloride while groups B1–6 were treated with 0.5 μM acetylcholine chloride only. The results showed that the intracellular fluorescence in A group increased but decreased in B group indicating the increase of intracellular free calcium. Such a changing of intracellular fluorescence suggested the stimulation effect of acetylcholine chloride on opening of the calcium channel which led to flow in of extracellular calcium ion. In two groups of lambs with different age, injection of acetylcholine chloride changed the blood pressure from baseline levels in age-dose-dependent way. Besides, renal blood flow and renal vascular resistance were also changed both age dependent and dose dependent. In an endothelial function study, the subjects treated with 11 mM acetylcholine chloride with 4 protocols. In protocol 2, acetylcholine chloride significantly increased the cutaneous vascular conductance (CVC). In protocol 3, inter-site and interday CV below 35% were observed for acetylcholine chloride–CVCpeak, which was increased in protocol 4 as well as the increasing of CVC values, suggesting acetylcholine chloride is reliable to study the endothelial function.

• 动物研究
  剂量	Dog: 0.001 mg/kg - 1 mg/kg[4] (i.v.) Mice: 20 mg/kg[5] (s.c.)
  给药途径	i.v., s.c.