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编 号：F167890
分子式：C22H15N2NaO7S2
分子量：506.48
分子式：C22H15N2NaO7S2
分子量：506.48
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CAS No: 14541-90-3
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生物活性：
Uniblue A sodium is a reactive protein stain that can be used in the covalent pre-gel staining of the protein (Ex=594 nM).
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Prepare the derivatization buffer: 100 mM NaHCO3, 10% SDS, pH 8-9.2. Add 10 μL of 200 mM Uniblue A solution (dissolved in derivatization buffer) to 90 μL protein solution.3. The samples are heated at 100°C for 1 min to perform the staining. 4. Add 100 μL of reducing solution composed of 10% glycerol and 20 mM dithiotreitol (DTT) in 200 mM Tris buffer with pH 6.8. 5. Excess Uniblue A reacts with Tris, resulting in a blue compound, serving as running front indicator in electrophoresis.6. The sample is heated another minute at 100°C in order to achieve efficient reduction and allowed to cool to room temperature. 7. Add 20 μL alkylation solution containing 550 mM iodoacetamide (IAA), incubated for 5 minutes.8. the samples are subjected to SDS-PAGE.Note: Dry protein samples or samples in compatible buffers (i.e. free of amines) can be diluted directly with the derivatization buffer solution to 5 mg/mL. In other cases, a prior trichloroacetic acid (TCA)/acetone precipitation or buffer exchange by ultrafiltration is recommended (see below).As positive control, bovine serum albumin (BSA) in derivatization buffer is used at a concentration of 10 mg/mL.
Uniblue A sodium is a reactive protein stain that can be used in the covalent pre-gel staining of the protein (Ex=594 nM).
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Prepare the derivatization buffer: 100 mM NaHCO3, 10% SDS, pH 8-9.2. Add 10 μL of 200 mM Uniblue A solution (dissolved in derivatization buffer) to 90 μL protein solution.3. The samples are heated at 100°C for 1 min to perform the staining. 4. Add 100 μL of reducing solution composed of 10% glycerol and 20 mM dithiotreitol (DTT) in 200 mM Tris buffer with pH 6.8. 5. Excess Uniblue A reacts with Tris, resulting in a blue compound, serving as running front indicator in electrophoresis.6. The sample is heated another minute at 100°C in order to achieve efficient reduction and allowed to cool to room temperature. 7. Add 20 μL alkylation solution containing 550 mM iodoacetamide (IAA), incubated for 5 minutes.8. the samples are subjected to SDS-PAGE.Note: Dry protein samples or samples in compatible buffers (i.e. free of amines) can be diluted directly with the derivatization buffer solution to 5 mg/mL. In other cases, a prior trichloroacetic acid (TCA)/acetone precipitation or buffer exchange by ultrafiltration is recommended (see below).As positive control, bovine serum albumin (BSA) in derivatization buffer is used at a concentration of 10 mg/mL.
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