产品
编 号：F157463
分子式：C35H40N4O4
分子量：580.72
分子式：C35H40N4O4
分子量：580.72
产品类型
规格
价格
是否有货
1mg
询价
询价
5mg
询价
询价
结构图
CAS No: 142234-85-3
产品详情
生物活性：
N-Methylmesoporphyrin IX (NMM), a widely used G-quadruplex DNA specific fluorescent binder, is an efficient probe for monitoring Aβ fibrillation. N-Methylmesoporphyrin IX is an in situ inhibitor and an ex situ monitor for Aβ amyloidogenesis both in vitro and in cells. N-Methylmesoporphyrin IX is sensitive to G-quadruplexes DNA but has no response to duplexes, triplexes and single-stranded forms DNA. N-Methylmesoporphyrin IX is nonfluorescent alone or in monomeric Aβ environments, but emits strong fluorescence through stacking with the Aβ assemblies.
体外研究：
By use of N-Methylmesoporphyrin IX (NMM) as an ex situ probe, NMM is added into the incubated Aβ40 solution. The concentration of NMM is fixed at 1μM. To examine the influence of Rhodamine B, 1 μM NMM with 0.1 μM or 0.5 μM Rhodamine B along with 10μM Aβ40 are measured. In the study of using NMM as an in situ inhibitor, NMM (10 μM) is co-incubated with Aβ40 (50 μM) for 7 days at 37 °C. Additional NMM is added before PL measurement to make the concentration of NMM constant (1 μM). The fluorescence spectra of NMM are collected from 550 to 700 nm with an excitation wavelength of 399 nm.N-Methylmesoporphyrin IX (NMM) is sensitive to G-quadruplexes DNA but has no response to duplexes, triplexes and single-stranded forms DNA. Upon binding to quadruplex DNA, for efficient π-π stacking, NMM can adjust its macrocycle geometry to match the terminal face of a G-quadruplex, leading to an enhancement in its fluorescence.
N-Methylmesoporphyrin IX (NMM), a widely used G-quadruplex DNA specific fluorescent binder, is an efficient probe for monitoring Aβ fibrillation. N-Methylmesoporphyrin IX is an in situ inhibitor and an ex situ monitor for Aβ amyloidogenesis both in vitro and in cells. N-Methylmesoporphyrin IX is sensitive to G-quadruplexes DNA but has no response to duplexes, triplexes and single-stranded forms DNA. N-Methylmesoporphyrin IX is nonfluorescent alone or in monomeric Aβ environments, but emits strong fluorescence through stacking with the Aβ assemblies.
体外研究：
By use of N-Methylmesoporphyrin IX (NMM) as an ex situ probe, NMM is added into the incubated Aβ40 solution. The concentration of NMM is fixed at 1μM. To examine the influence of Rhodamine B, 1 μM NMM with 0.1 μM or 0.5 μM Rhodamine B along with 10μM Aβ40 are measured. In the study of using NMM as an in situ inhibitor, NMM (10 μM) is co-incubated with Aβ40 (50 μM) for 7 days at 37 °C. Additional NMM is added before PL measurement to make the concentration of NMM constant (1 μM). The fluorescence spectra of NMM are collected from 550 to 700 nm with an excitation wavelength of 399 nm.N-Methylmesoporphyrin IX (NMM) is sensitive to G-quadruplexes DNA but has no response to duplexes, triplexes and single-stranded forms DNA. Upon binding to quadruplex DNA, for efficient π-π stacking, NMM can adjust its macrocycle geometry to match the terminal face of a G-quadruplex, leading to an enhancement in its fluorescence.
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