产品
编 号：F149835
分子式：C16H22N4O4S
分子量：366.44
分子式：C16H22N4O4S
分子量：366.44
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CAS No: 139554-72-6
产品详情
生物活性：
Biotin-C2-maleimide is an antibody conjugate reagent, can bind to many biomolecules without significantly changing the biological activity of the target molecule.
体外研究：
Biotin-C2-maleimide can act bioconjugation with 5 eq. SUMO-1 probes.1. Biotin C2 maleimide (ATT Bioquest, dissolved in DMSO) is performed in Tris buffer (50 mM Tris, 300 mM NaCl; pH 7.0) after 15 min reduction with Tris (2-carboxyethyl)phosphine (TCEP, 120 μM) at room temperature.2. After 1 h incubation, the unreacted Biotin is separated by Ni-NTA affinity chromatography followed by dialysis against PBS buffer (pH 7.4).
Biotin-C2-maleimide is an antibody conjugate reagent, can bind to many biomolecules without significantly changing the biological activity of the target molecule.
体外研究：
Biotin-C2-maleimide can act bioconjugation with 5 eq. SUMO-1 probes.1. Biotin C2 maleimide (ATT Bioquest, dissolved in DMSO) is performed in Tris buffer (50 mM Tris, 300 mM NaCl; pH 7.0) after 15 min reduction with Tris (2-carboxyethyl)phosphine (TCEP, 120 μM) at room temperature.2. After 1 h incubation, the unreacted Biotin is separated by Ni-NTA affinity chromatography followed by dialysis against PBS buffer (pH 7.4).
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