产品
编 号：F126199
分子式：C47H47ClN2O3S
分子量：755.41
分子式：C47H47ClN2O3S
分子量：755.41
产品类型
规格
价格
是否有货
50mg
160
In-stock
100mg
256
In-stock
250mg
480
In-stock
结构图
CAS No: 134127-48-3
产品详情
生物活性：
IR 813 tosylate is a near-infrared (NIR) fluorescent dye (λex=815 nm, λem=840 nm) and can be used for visualizing regional lymph nodes in mice.
体内研究：
IR 813 tosylate displays a maximum fluorescence intensity at 4 h postinjection together with a rapid extravasation, when being used for visualizing regional lymph nodes in mice.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Animal: Ten-week-old female Balb/cOlaAnN mice are kept in a 12 h light/dark cycle to reduce tissue autofluorescence in the NIR region, and had access to food and water ad libitum.2. Dose: A single dose 5.1 nmol of IR 813 dye (20 μL of 0.173 mg/mL, dissolved in a PEG-400/ethanol/water=3:2:5, v/v/v solution) is subcutaneous injected in the right anterior paw of mice.3. Imaging: Using the Fluobeam700 NIR imaging system to perform in vivo optical imaging (739 nm excitation light (3.5 mW), 750 nm long-pass emission cutoff filter).Fluorescence intensity in the axillary lymph node (ALN) is recorded for 1 week (5 min, 1 h, 4 h, 24 h, 7 days).4. Data analysis: Semiquantitative data is obtained from the fluorescence images using ImageJ 1.44 software.
体外研究：
IR 813 (17.3 μM, 2 h) tosylate induces a 31.4% hemolysis in red blood cells.IR 813 (5.9 μM, 24 h) tosylate induces 50% cell death in MRC-5 cells.
IR 813 tosylate is a near-infrared (NIR) fluorescent dye (λex=815 nm, λem=840 nm) and can be used for visualizing regional lymph nodes in mice.
体内研究：
IR 813 tosylate displays a maximum fluorescence intensity at 4 h postinjection together with a rapid extravasation, when being used for visualizing regional lymph nodes in mice.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Animal: Ten-week-old female Balb/cOlaAnN mice are kept in a 12 h light/dark cycle to reduce tissue autofluorescence in the NIR region, and had access to food and water ad libitum.2. Dose: A single dose 5.1 nmol of IR 813 dye (20 μL of 0.173 mg/mL, dissolved in a PEG-400/ethanol/water=3:2:5, v/v/v solution) is subcutaneous injected in the right anterior paw of mice.3. Imaging: Using the Fluobeam700 NIR imaging system to perform in vivo optical imaging (739 nm excitation light (3.5 mW), 750 nm long-pass emission cutoff filter).Fluorescence intensity in the axillary lymph node (ALN) is recorded for 1 week (5 min, 1 h, 4 h, 24 h, 7 days).4. Data analysis: Semiquantitative data is obtained from the fluorescence images using ImageJ 1.44 software.
体外研究：
IR 813 (17.3 μM, 2 h) tosylate induces a 31.4% hemolysis in red blood cells.IR 813 (5.9 μM, 24 h) tosylate induces 50% cell death in MRC-5 cells.
产品资料
Copyright©2015-2024 沪ICP备2022026524号-1
沪公网安备