产品
编 号：F119886
分子式：C18H10K4N2O11
分子量：586.67
分子式：C18H10K4N2O11
分子量：586.67
产品类型
规格
价格
是否有货
结构图
CAS No: 132319-57-4
产品详情
生物活性：
Mag-Fura-2 tetrapotassium is a UV excitable rational fluorescent Mg2+/Ca2+ indicator (Ex=334-360 nm, Em=510 nm). Mag-Fura-2 tetrapotassium can be used for the determination of Mg2+ and Ca2+ concentrations.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Monitoring of Ca2+ release:1. Incubate dispersed parotid acinar cells with 8 μM Mag-fura-2 tetrapotassium for 30 min at 37℃. (acinar cells for example).2. Wash cells twice with fresh HBSS-H without BSA.3. Precoate cell adhesive Cell-Tak on sample chambers.4. Transfer the dye-loaded cells to the chambers and attach to the bottom.5. Mounte the sample chambers on the stage of an inverted microscope (equipped with a 40 × objective), wash with BSA-free HBSS-H and then with Mg2+/ATP-free ICM.6. Incubate acinar cells with Mg2+/ATP-free ICM containing 50 μg/mL saponin for 3-5 min at room temperature.7. Wash the cells with ICM containing Mg2+ and ATP, and incubate in the complete ICM for at least 5 min to allow complete filling of the intracellular Ca2+ stores.8. Alternately excite permeabilised cells, capture and digitise fluorescence emission at 510 nm by a digital imaging system (record the 344 nm/360 nm ratio every 20 s).Note: ICM (intracellular-like medium) containing 125 mM KCl, 19 mM NaCl, 10 mM HEPES (pH 7.3 with KOH), 3 mM ATP, 1.4 mM MgCl2, 0.33 mM CaCl2, and 1 mM EGTA.
Mag-Fura-2 tetrapotassium is a UV excitable rational fluorescent Mg2+/Ca2+ indicator (Ex=334-360 nm, Em=510 nm). Mag-Fura-2 tetrapotassium can be used for the determination of Mg2+ and Ca2+ concentrations.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Monitoring of Ca2+ release:1. Incubate dispersed parotid acinar cells with 8 μM Mag-fura-2 tetrapotassium for 30 min at 37℃. (acinar cells for example).2. Wash cells twice with fresh HBSS-H without BSA.3. Precoate cell adhesive Cell-Tak on sample chambers.4. Transfer the dye-loaded cells to the chambers and attach to the bottom.5. Mounte the sample chambers on the stage of an inverted microscope (equipped with a 40 × objective), wash with BSA-free HBSS-H and then with Mg2+/ATP-free ICM.6. Incubate acinar cells with Mg2+/ATP-free ICM containing 50 μg/mL saponin for 3-5 min at room temperature.7. Wash the cells with ICM containing Mg2+ and ATP, and incubate in the complete ICM for at least 5 min to allow complete filling of the intracellular Ca2+ stores.8. Alternately excite permeabilised cells, capture and digitise fluorescence emission at 510 nm by a digital imaging system (record the 344 nm/360 nm ratio every 20 s).Note: ICM (intracellular-like medium) containing 125 mM KCl, 19 mM NaCl, 10 mM HEPES (pH 7.3 with KOH), 3 mM ATP, 1.4 mM MgCl2, 0.33 mM CaCl2, and 1 mM EGTA.
产品资料
Copyright©2015-2024 沪ICP备2022026524号-1
沪公网安备