产品
编 号：F119850
分子式：C21H14K4N2O9
分子量：594.74
分子式：C21H14K4N2O9
分子量：594.74
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CAS No: 132299-21-9
产品详情
生物活性：
Mag-Indo-1 tetrapotassium salt is a cell impermeable fluorescent probe for Mg2+ detection.
体外研究：
Mag-Indo-1 tetrapotassium salt can be used to determine the free Mg2+ concentration in the cytoplasmic solutions.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Calibrating solutions containing various concentrations of Mg2+ are prepared by mixing different ratios of the two stock solutions: one contains (mM) 150 KCl, 0.1 EGTA and 5 Hepes (pH 7.2 with KOH), and the other contains 100 MgCl2, 0.1 EGTA and 5 Hepes (pH 7.2 with KOH). 2. The calibration curve is constructed using calibrating solutions containing 1 μM Mag-Indo-1 tetrapotassium salt. 3. The ‘Mg2+’ calibrating solution contained (mM): 130 KCl, 4 EDTA and 5 Hepes (pH 7.2 with KOH). 4. The relationship between the background-corrected value of the fluorescence ratio (R) and the Mg2+ concentration is fitted with the following theoretical equation. [Mg]=K (R-Rmin)/(Rmax-R), where [Mg] is the concentration of free Mg2+ ion, Rmin is the R value at 0 [Mg2+], and Rmax is the R value at saturating Mg2+. The curve fitting gave Rmin= 0.053, Rmax= 1.57, and K= 5.1 mM. Note: the specific curve can refer to the reference.
Mag-Indo-1 tetrapotassium salt is a cell impermeable fluorescent probe for Mg2+ detection.
体外研究：
Mag-Indo-1 tetrapotassium salt can be used to determine the free Mg2+ concentration in the cytoplasmic solutions.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Calibrating solutions containing various concentrations of Mg2+ are prepared by mixing different ratios of the two stock solutions: one contains (mM) 150 KCl, 0.1 EGTA and 5 Hepes (pH 7.2 with KOH), and the other contains 100 MgCl2, 0.1 EGTA and 5 Hepes (pH 7.2 with KOH). 2. The calibration curve is constructed using calibrating solutions containing 1 μM Mag-Indo-1 tetrapotassium salt. 3. The ‘Mg2+’ calibrating solution contained (mM): 130 KCl, 4 EDTA and 5 Hepes (pH 7.2 with KOH). 4. The relationship between the background-corrected value of the fluorescence ratio (R) and the Mg2+ concentration is fitted with the following theoretical equation. [Mg]=K (R-Rmin)/(Rmax-R), where [Mg] is the concentration of free Mg2+ ion, Rmin is the R value at 0 [Mg2+], and Rmax is the R value at saturating Mg2+. The curve fitting gave Rmin= 0.053, Rmax= 1.57, and K= 5.1 mM. Note: the specific curve can refer to the reference.
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