产品
编 号：F118530
分子式：C45H81N13O14S
分子量：1060.27
分子式：C45H81N13O14S
分子量：1060.27
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规格
价格
是否有货
1mg
询价
询价
5mg
询价
询价
10mg
询价
询价
50mg
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询价
结构图
CAS No: 131602-53-4
产品详情
生物活性：
β-Amyloid (25-35) (Amyloid beta-peptide (25-35)) is the fragment Aβ(25-35) of the Alzheimer's amyloid β-peptide, has shown neurotoxic activities in cultured cells.
体内研究：
β-Amyloid (25-35) 可用于动物建模，构建阿尔茨海默病模型。β-Amyloid (25-35) 可用于动物建模，构建阿尔茨海默病模型。致病原理β-Amyloid 大量沉积，使神经元内的 Ca2+ 浓度升高，而 Ca2+ 又参与调控Tau 蛋白磷酸化，Tau 蛋白的过度磷酸化造成了神经纤维缠结和神经元死亡，最后动物逐渐出现认知症状，并发展为阿尔茨海默病。 具体造模方法Rat: Wistar • male • 180-200 gAdministration: 3 μg/μl in 5 μl within 1.2 μl/min • i.c.v • once Note(1) 动物饲养在规定的环境中 (23±1°C，50%±2%湿度)，光照/黑暗周期为12小时 (光照在上午8:00–晚上8:00)。(2) 使用汉密尔顿注射器将 G27 号针头插入深度 3.8 毫米进行注射。造模成功指标分子变化：Aβ1-40蛋白增加； 丙二醛 (MDA) 增加； 海马 CA1 区 AChE 和 Bax 表达减少。认知变化：莫里斯水迷宫测试学习和记忆缺陷。同类产品: 拮抗产品:Aducanumab (HY-P9967)Donanemab (HY-P99859)
体外研究：
The amino acid sequence of Aβ(25-35) peptide is NH2-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-COOH, where the first Gly represents the amino acid 25 and the last Met represents the amino acid 35. Amyloid beta-peptide(25-35) is also investigated in gel state for the first time. Comparative studies are also carried out using vibrational absorption and ECD. The conformational preference of Aβ(25-35) peptide film is also investigated using vibrational absorption and VCD spectroscopy. Amyloid beta-peptide(25-35) induces apoptotic effects on isolated brain mitochondria and the redox state of methionine-35, plays a key role in the induction of programmed cellular death pathways and toxic events.β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure.2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃.3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.Methods vary depends on the downstream applications.
β-Amyloid (25-35) (Amyloid beta-peptide (25-35)) is the fragment Aβ(25-35) of the Alzheimer's amyloid β-peptide, has shown neurotoxic activities in cultured cells.
体内研究：
β-Amyloid (25-35) 可用于动物建模，构建阿尔茨海默病模型。β-Amyloid (25-35) 可用于动物建模，构建阿尔茨海默病模型。致病原理β-Amyloid 大量沉积，使神经元内的 Ca2+ 浓度升高，而 Ca2+ 又参与调控Tau 蛋白磷酸化，Tau 蛋白的过度磷酸化造成了神经纤维缠结和神经元死亡，最后动物逐渐出现认知症状，并发展为阿尔茨海默病。 具体造模方法Rat: Wistar • male • 180-200 gAdministration: 3 μg/μl in 5 μl within 1.2 μl/min • i.c.v • once Note(1) 动物饲养在规定的环境中 (23±1°C，50%±2%湿度)，光照/黑暗周期为12小时 (光照在上午8:00–晚上8:00)。(2) 使用汉密尔顿注射器将 G27 号针头插入深度 3.8 毫米进行注射。造模成功指标分子变化：Aβ1-40蛋白增加； 丙二醛 (MDA) 增加； 海马 CA1 区 AChE 和 Bax 表达减少。认知变化：莫里斯水迷宫测试学习和记忆缺陷。同类产品: 拮抗产品:Aducanumab (HY-P9967)Donanemab (HY-P99859)
体外研究：
The amino acid sequence of Aβ(25-35) peptide is NH2-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-COOH, where the first Gly represents the amino acid 25 and the last Met represents the amino acid 35. Amyloid beta-peptide(25-35) is also investigated in gel state for the first time. Comparative studies are also carried out using vibrational absorption and ECD. The conformational preference of Aβ(25-35) peptide film is also investigated using vibrational absorption and VCD spectroscopy. Amyloid beta-peptide(25-35) induces apoptotic effects on isolated brain mitochondria and the redox state of methionine-35, plays a key role in the induction of programmed cellular death pathways and toxic events.β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure.2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃.3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.Methods vary depends on the downstream applications.
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