产品
编 号:F763059
分子式:C16H24N6O4
分子量:364.4
产品类型
结构图
CAS No: 1064123-31-4
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产品详情
生物活性:
LysoTracker Yellow HCK 123 is a potent yellow membrane-permeable fluorescent probe. LysoTracker Yellow HCK 123 is a weakly basic amine that selectively accumulates in cellular compartments with low luminal pH. (λex=465 nm, λem=535 nm).
体外研究:
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).LysoTracker Yellow HCK 123 assay (to quantify GAPDH):1. Culture EMT6 cells in 8-well cell culture slides. Incubate the cells according to your normal protocol.2. Add TONS504 per well to make the final concentration at 30 μg/mL.Incubate cells at 37 °C for 4 hours.3. Add LysoTracker Yellow HCK 123 per well to make the final concentration at 50 μM. Incubate cells at 37 °C, 5% CO2, for 30 minutes.4. Analyze samples on a confocal laser scanning microscope.