产品
编 号：F760674
分子式：C31H36BF2N5O5
分子量：607.46
分子式：C31H36BF2N5O5
分子量：607.46
产品类型
规格
价格
是否有货
结构图
CAS No: 849464-08-0
产品详情
生物活性：
Bodipy TMR-X muscimol is a Bodipy labeled Muscimol (HY-N2313) (Ex=543 nm, Em=572 nm). Muscimol is a GABAA agonist. Bodipy TMR-X muscimol can be used for imaging the spread of reversible brain inactivations.
体内研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Rats are infused with Bodipy TMR-X muscimol as below: dilute the stock solution to 2 μg/μL (in PBS), infused into both hemispheres at a rate of 0.25 μL/min for a single minute, resulting in a final infusion volume of 0.25 μL and a final dose of 0.5 μg per side. 2. The animals are sacrificed by rapid decapitation 15 min after infusionin order to match the spread to what the experimental animals received immediately prior to behavioral testing. 3. The brains are removed and flash frozen in45°C isopentane, stored at80°C. 4. Coronal slices are sectioned at 60 μm. The slices are mounted on charged microscope slides and counterstained with DAPI. 5. The stained slices are incubated in a cool, dark room at room temperature for 3 d before being visualized with a confocal microscope. 6. A digital plate from the Paxinos and Watson (2007) rat brain atlas is overlaid on the image to visualize the spread.
Bodipy TMR-X muscimol is a Bodipy labeled Muscimol (HY-N2313) (Ex=543 nm, Em=572 nm). Muscimol is a GABAA agonist. Bodipy TMR-X muscimol can be used for imaging the spread of reversible brain inactivations.
体内研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Rats are infused with Bodipy TMR-X muscimol as below: dilute the stock solution to 2 μg/μL (in PBS), infused into both hemispheres at a rate of 0.25 μL/min for a single minute, resulting in a final infusion volume of 0.25 μL and a final dose of 0.5 μg per side. 2. The animals are sacrificed by rapid decapitation 15 min after infusionin order to match the spread to what the experimental animals received immediately prior to behavioral testing. 3. The brains are removed and flash frozen in45°C isopentane, stored at80°C. 4. Coronal slices are sectioned at 60 μm. The slices are mounted on charged microscope slides and counterstained with DAPI. 5. The stained slices are incubated in a cool, dark room at room temperature for 3 d before being visualized with a confocal microscope. 6. A digital plate from the Paxinos and Watson (2007) rat brain atlas is overlaid on the image to visualize the spread.
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