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编 号：F759270
分子式：C21H13Br5N2O3
分子量：740.86
分子式：C21H13Br5N2O3
分子量：740.86
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CAS No: 623903-26-4
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生物活性：
Tetrabromorhodamine 123 (TBR) bromide is a photosensitizer. Tetrabromorhodamine 123 bromide can be used for the research of photo dynamic therapy (PDT) and cancer.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).A. Labeling of Cells:1.Incubate the cells according to your normal protocol.2. Cells are incubatedwith DMEM containing 5 μM TBR and maintained in the dark in a CO2 incubator at 37℃.3. After 1 h, chang the medium to normal culture medium without phenol red, and cells were exposed to visible light with a 500 W Xe arc with a filter.B. The intracellular localization of TBR:1. Cells are cultured in 35 mm diameter glassbottomed dishes for 48 h. Incubate the cells according to your normal protocol.2. For triple-staining, medium is changed to a solution containing BODIPY-TR ceramide (5 μM).3. Cells areincubated at 48℃ for 20 min and then further incubated at 37℃ for 1 h, afterwhich the solution is changed to normal medium containing 5 μM TBR.4. The stock solution (1 mM) of Hoechst 33342 was added to the medium (final concentration, 100 μM), and cells were incubated for 15 min.5. Cells are washed with phosphate-buffered saline (plus Ca2+, Mg2+) and then observed with the aid of confocal laser scanning microscopy (LSM).
Tetrabromorhodamine 123 (TBR) bromide is a photosensitizer. Tetrabromorhodamine 123 bromide can be used for the research of photo dynamic therapy (PDT) and cancer.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).A. Labeling of Cells:1.Incubate the cells according to your normal protocol.2. Cells are incubatedwith DMEM containing 5 μM TBR and maintained in the dark in a CO2 incubator at 37℃.3. After 1 h, chang the medium to normal culture medium without phenol red, and cells were exposed to visible light with a 500 W Xe arc with a filter.B. The intracellular localization of TBR:1. Cells are cultured in 35 mm diameter glassbottomed dishes for 48 h. Incubate the cells according to your normal protocol.2. For triple-staining, medium is changed to a solution containing BODIPY-TR ceramide (5 μM).3. Cells areincubated at 48℃ for 20 min and then further incubated at 37℃ for 1 h, afterwhich the solution is changed to normal medium containing 5 μM TBR.4. The stock solution (1 mM) of Hoechst 33342 was added to the medium (final concentration, 100 μM), and cells were incubated for 15 min.5. Cells are washed with phosphate-buffered saline (plus Ca2+, Mg2+) and then observed with the aid of confocal laser scanning microscopy (LSM).
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