产品
编 号：F755513
分子式：C26H31ClN4O5S
分子量：547.07
分子式：C26H31ClN4O5S
分子量：547.07
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CAS No: 2758364-03-1
产品详情
生物活性：
Rf470DL is a rotor-fluorogenic D-amino acid (RfDAA). Rf470DL can be used for labeling bacteria (Ex=470 nm, Em=640 nm).
体外研究：
Rf470DL can realize ash-free imaging of bacterial cell walls.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. RfDAA/FDAA stock solutions are prepared in DMSO at a concentration of 100 mM and stored at -20°C before use. 2.1 For long-pulse labeling of B. subtilis and E. coli, dilute the exponential phase cultures with fresh LB broth containing 1 mM Rf470DL to OD600~0.05, and incubate for 1 h. 2.2 Image immediately using a Nikon Ti-E inverted microscopy system without washing and fixation.3.1 For short-pulse labeling of S. venezuelae, Rf470DL stock solution is added directly to exponential phase cultures to a final concentration of 0.5 mM, followed by incubating at 30 °C with shaking for 15 min. 3.2 Image immediately using a Nikon Ti-E inverted microscopy system without washing and fixation.
Rf470DL is a rotor-fluorogenic D-amino acid (RfDAA). Rf470DL can be used for labeling bacteria (Ex=470 nm, Em=640 nm).
体外研究：
Rf470DL can realize ash-free imaging of bacterial cell walls.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. RfDAA/FDAA stock solutions are prepared in DMSO at a concentration of 100 mM and stored at -20°C before use. 2.1 For long-pulse labeling of B. subtilis and E. coli, dilute the exponential phase cultures with fresh LB broth containing 1 mM Rf470DL to OD600~0.05, and incubate for 1 h. 2.2 Image immediately using a Nikon Ti-E inverted microscopy system without washing and fixation.3.1 For short-pulse labeling of S. venezuelae, Rf470DL stock solution is added directly to exponential phase cultures to a final concentration of 0.5 mM, followed by incubating at 30 °C with shaking for 15 min. 3.2 Image immediately using a Nikon Ti-E inverted microscopy system without washing and fixation.
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