产品
编 号：F010613
分子式：C16H16N2S
分子量：268.38
分子式：C16H16N2S
分子量：268.38
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CAS No: 10205-62-6
产品详情
生物活性：
BTA-2, a benzothiazole dye, is structurally similar to thioflavin T (ThT), which exhibits an enhanced fluorescence signal when bound to amyloid fibrils. BTA-2 has distinct absorption and emission characteristics in solution and when bound to amyloid fibrils, which makes it can used for identifying amyloid fibrils using spectroscopy.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).To research how CH3CN affects binding of amyloid fibrils (varied the amount of CH3CN but maintained the same concentration of BTA-2, fibrils, and water) and how the concentration of BTA-2 affects binding (varied the amount of BTA-2 but had a constant amount of CH3CN, fibrils, and water) .BTA-2 in solution and in the presence of amyloid fibrils: 1. Insulin obtains from a bovine pancreas, and is stored at ?20°C in a desiccator.2. To synthesize BTA-2 and further purified.3. To prepare fibrils: Insulin is dissolved in pH 2 water (5 mg/mL) and filters through a 0.2 μmfilter, heats at 60℃ for 24 h.Centrifuge at 3000 rpm for 2.5 min to remove any globular artifacts. The supernatant containing the fibrils is saved for later use. Solutions are stored in a refrigerator (10℃) until needed.4. BTA-2/fibrils solutions (are prepared in two ways): (First way) Diluting (100 μL) of the stock fibril solution with 880 μL of pH 2 water, adding (20 μL) of 0.8 mg/mL (3.15 mM) BTA-2 in CH3CN. (Second way) Diluting (100 μL) of the stock fibrilsolution with a 1:1 mixture of nanopure water: CH3CN, adding (20 μL) of 0.8 mg/mL (3.15 mM) BTA-2.5. Two similar solutions are made that contained only the BTA-2 without the fibrils.6. The standard amyloid fluorescent marker is thioflavin T (ThT): 20 μL of 0.8 mg/mL (2.51 mM) ThT in CH3CN and 100 μL of stock fibril solutions is diluted with 880 μL of pH 2 water.7. The emission spectra of BTA-2 in pH 2 water excited at 430 nm and excited at 360 nm, respectively.
BTA-2, a benzothiazole dye, is structurally similar to thioflavin T (ThT), which exhibits an enhanced fluorescence signal when bound to amyloid fibrils. BTA-2 has distinct absorption and emission characteristics in solution and when bound to amyloid fibrils, which makes it can used for identifying amyloid fibrils using spectroscopy.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).To research how CH3CN affects binding of amyloid fibrils (varied the amount of CH3CN but maintained the same concentration of BTA-2, fibrils, and water) and how the concentration of BTA-2 affects binding (varied the amount of BTA-2 but had a constant amount of CH3CN, fibrils, and water) .BTA-2 in solution and in the presence of amyloid fibrils: 1. Insulin obtains from a bovine pancreas, and is stored at ?20°C in a desiccator.2. To synthesize BTA-2 and further purified.3. To prepare fibrils: Insulin is dissolved in pH 2 water (5 mg/mL) and filters through a 0.2 μmfilter, heats at 60℃ for 24 h.Centrifuge at 3000 rpm for 2.5 min to remove any globular artifacts. The supernatant containing the fibrils is saved for later use. Solutions are stored in a refrigerator (10℃) until needed.4. BTA-2/fibrils solutions (are prepared in two ways): (First way) Diluting (100 μL) of the stock fibril solution with 880 μL of pH 2 water, adding (20 μL) of 0.8 mg/mL (3.15 mM) BTA-2 in CH3CN. (Second way) Diluting (100 μL) of the stock fibrilsolution with a 1:1 mixture of nanopure water: CH3CN, adding (20 μL) of 0.8 mg/mL (3.15 mM) BTA-2.5. Two similar solutions are made that contained only the BTA-2 without the fibrils.6. The standard amyloid fluorescent marker is thioflavin T (ThT): 20 μL of 0.8 mg/mL (2.51 mM) ThT in CH3CN and 100 μL of stock fibril solutions is diluted with 880 μL of pH 2 water.7. The emission spectra of BTA-2 in pH 2 water excited at 430 nm and excited at 360 nm, respectively.
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