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编 号：F749704
分子式：C40H46BF4N7O
分子量：727.64
分子式：C40H46BF4N7O
分子量：727.64
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CAS No: 2183473-57-4
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生物活性：
Cy3 methyltetrazine (TZ-Cy3) is a click chemistry reagent with methyltetrazine building blocks that is highly reactive towards cyclooctene. Cy3 methyltetrazine is also a tetrazine-modified fluorescent probe that can be used to analyze protein phosphorylation in solution and living cells.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Confocal imaging of phosphorylation in cells:1. Culture H1299 cells in a coverglass-bottom imaging dish for 24 h (take H1299 cells as an example).2. Incubate H1299 cells with different concentrations of ATP-NB (a ATP analogue functionalized by norbornene, which can penetrate cells and efficiently phosphorylate proteins in living cells) for 1 h.3. Add Cy3 methyltetrazine to label the phosphorylated protein with fluorescence.4. Incubate with fresh medium for 1 h.5. Wash the cells twice with PBS to release the free Cy3 methyltetrazine probe.6.Incubate the cells with 4% paraformaldehyde for 15 min and with 0.1% Triton X-100 solution at room temperature for 10 min.7. Wash cells 4 times with PBS.8. Use a confocal microscopy imaging system to obtain the high-resolution images of cells (Ex=488 nm; Em=561 nm).
Cy3 methyltetrazine (TZ-Cy3) is a click chemistry reagent with methyltetrazine building blocks that is highly reactive towards cyclooctene. Cy3 methyltetrazine is also a tetrazine-modified fluorescent probe that can be used to analyze protein phosphorylation in solution and living cells.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Confocal imaging of phosphorylation in cells:1. Culture H1299 cells in a coverglass-bottom imaging dish for 24 h (take H1299 cells as an example).2. Incubate H1299 cells with different concentrations of ATP-NB (a ATP analogue functionalized by norbornene, which can penetrate cells and efficiently phosphorylate proteins in living cells) for 1 h.3. Add Cy3 methyltetrazine to label the phosphorylated protein with fluorescence.4. Incubate with fresh medium for 1 h.5. Wash the cells twice with PBS to release the free Cy3 methyltetrazine probe.6.Incubate the cells with 4% paraformaldehyde for 15 min and with 0.1% Triton X-100 solution at room temperature for 10 min.7. Wash cells 4 times with PBS.8. Use a confocal microscopy imaging system to obtain the high-resolution images of cells (Ex=488 nm; Em=561 nm).
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