产品
编 号：F749687
分子式：C50H53ClN4O2
分子量：777.43
分子式：C50H53ClN4O2
分子量：777.43
产品类型
规格
价格
是否有货
结构图
CAS No: 2182601-72-3
产品详情
生物活性：
Cy5 DBCO chloride is an azide reaction probe and the addition of DBCO molecules allows the imaging of azide-labelled biomolecules by a copper-free “Click Chemistry” reaction. Cy5 DBCO (chloride) is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
体外研究：
Cy5 DBCO chloride (2-50 μM) can control the extent of labelling by varying the concentration of DBCO-Cy5 in a dose-dependent manner, with the amount of DBCO-Cy5 bound at the cell surface increasing with increasing DBCO-Cy5 concentration.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1.Incubate the cells at a density of 3×104 cells in a cell culture dish for 3 days and then wash the cells twice with DPBS (pH 7.4). Incubate the cells according to your normal protocol.2.Incubate the cells with Cy5 DBCO chloride at a final concentration of 20 μM for 1 h at 37°C.3.After incubation, wash cells with DPBS (pH 7.4) and fix with formaldehyde-glutaraldehyde combination fixative for 15 min at room temperature.4.After fixation, wash cells twice with DPBS (pH 7.4). Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.
Cy5 DBCO chloride is an azide reaction probe and the addition of DBCO molecules allows the imaging of azide-labelled biomolecules by a copper-free “Click Chemistry” reaction. Cy5 DBCO (chloride) is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
体外研究：
Cy5 DBCO chloride (2-50 μM) can control the extent of labelling by varying the concentration of DBCO-Cy5 in a dose-dependent manner, with the amount of DBCO-Cy5 bound at the cell surface increasing with increasing DBCO-Cy5 concentration.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1.Incubate the cells at a density of 3×104 cells in a cell culture dish for 3 days and then wash the cells twice with DPBS (pH 7.4). Incubate the cells according to your normal protocol.2.Incubate the cells with Cy5 DBCO chloride at a final concentration of 20 μM for 1 h at 37°C.3.After incubation, wash cells with DPBS (pH 7.4) and fix with formaldehyde-glutaraldehyde combination fixative for 15 min at room temperature.4.After fixation, wash cells twice with DPBS (pH 7.4). Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.
产品资料
Copyright©2015-2024 沪ICP备2022026524号-1
沪公网安备