产品
编 号:F749595
分子式:C26H28BClF2N4O2S
分子量:544.85
产品类型
结构图
CAS No: 217190-24-4
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产品详情
生物活性:
BODIPY TR Cadaverine, a cadaverine derivative, is a red fluorescent dye. BODIPY TR Cadaverine can be used in a a highly sensitive and robust fluorescent displacement assay, which binds to native LPS strongly, specifically recognizing lipid A, and is competitively displaced by compounds displaying an affinity for lipid A.
体外研究:
BODIPY TR Cadaverine assay (to quantify autophagy in cells):Cells were incubated with 125 nM BODIPY TR Cadaverine for 10 minutes, washed four times with PBS and lysed in 10 mM Tris-Cl pH 8, 0.1 % Triton X-100. Fluorescence levels were read in a SPECTRAmax fluorimeter (Molecular Devices) (λex=588 nm, λem=616 nm). Fluorescence levels were normalized for cell number by adding 0.2 mM ethidium bromide and reading DNA fluorescence (λex=530 nm, λem=590 nm).