产品
编 号：F749040
分子式：C22H23Cl2NO3
分子量：420.33
分子式：C22H23Cl2NO3
分子量：420.33
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规格
价格
是否有货
结构图
CAS No: 2102418-90-4
产品详情
生物活性：
DDAO-C6 is a cridone ester derivative, highly specific fluorescence for detecting human serum albumin (HSA). DDAO-C6 acts as an enzymatic activatable near-infrared fluorescent probe in visually sensing endogenous lipase from gut microbes (Ex/Em=600/658 nm).
体内研究：
DDAO-C6 can be used to guide the rapid identification and cultivation of lipase active fungal strains from intestinal microbes in human feces, which is a potential technique for the biological investigation of intestinal microbes.
体外研究：
DDAO-C6 (10 μM; 30 min; 37 ℃) shows a linear relationship between the fluorescence intensities and lipase concentrations (0-50 μg/mL), and products a red fluorescence signal in the presence of active lipase.DDAO-C6 hydrolysates have fluorescence properties, with excitation wavelength=550-600 nm, emission spectrum=630-700 nm.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Labeling of Intestinal Fungi:1. Disperse fresh intestinal secretions in sterile water and coate on a potato agar plate, containing penicillin (100 U/mL)/streptomycin (0.1 mg/mL) to inhibit intestinal bacteria. 2. Culture at 32 °C for about 5 days until the development of obvious fungal colonies. 3. Spray DDAO-C6 (100 μM) on colonies and incubate at 32 °C for 2 h. 4. Image plate samples on a Amersham Typhoon RGB (λex = 635 nm, λem = 670 ± 15 nm).
DDAO-C6 is a cridone ester derivative, highly specific fluorescence for detecting human serum albumin (HSA). DDAO-C6 acts as an enzymatic activatable near-infrared fluorescent probe in visually sensing endogenous lipase from gut microbes (Ex/Em=600/658 nm).
体内研究：
DDAO-C6 can be used to guide the rapid identification and cultivation of lipase active fungal strains from intestinal microbes in human feces, which is a potential technique for the biological investigation of intestinal microbes.
体外研究：
DDAO-C6 (10 μM; 30 min; 37 ℃) shows a linear relationship between the fluorescence intensities and lipase concentrations (0-50 μg/mL), and products a red fluorescence signal in the presence of active lipase.DDAO-C6 hydrolysates have fluorescence properties, with excitation wavelength=550-600 nm, emission spectrum=630-700 nm.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Labeling of Intestinal Fungi:1. Disperse fresh intestinal secretions in sterile water and coate on a potato agar plate, containing penicillin (100 U/mL)/streptomycin (0.1 mg/mL) to inhibit intestinal bacteria. 2. Culture at 32 °C for about 5 days until the development of obvious fungal colonies. 3. Spray DDAO-C6 (100 μM) on colonies and incubate at 32 °C for 2 h. 4. Image plate samples on a Amersham Typhoon RGB (λex = 635 nm, λem = 670 ± 15 nm).
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