产品
编 号：F748227
分子式：C24H24Cl2N2O
分子量：427.37
分子式：C24H24Cl2N2O
分子量：427.37
产品类型
规格
价格
是否有货
1mg
询价
询价
5mg
询价
询价
结构图
CAS No: 199116-50-2
产品详情
生物活性：
MitoTracker Orange CMTMRos is a fluorescent dye that labels mitochondria within live cells utilizing the mitochondrial membrane potential (Ex/Em: 551/576 nm).
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Cells: For live cells, follow Steps 1–7. For fixed cells, follow Steps 8–13. 1. Prepare a labeling solution at 25–500 nM in the desired cell culture medium, and warm it to the cell culture temperature. 2. Aspirate the culture medium from cells grown on coverslips. 3. Immerse the cells in the labeling medium for 15–45 min at 37?C. 4. Aspirate the labeling medium, and rinse the cells three times with culture medium. 5. Mount the cells. 6. Check the cells using a fluorescence microscope. If the fluorescence staining is too low, try one of the following options: i. Incubate for an additional 30 min in the normal culture medium to allow the thiol conjugation to proceed. ii. Try a higher initial concentration of the labeling solution. 7. When the fluorescence is sufficient, image the cells. 8. Carry out Steps 1–4, but use a labeling solution at a working concentration between 100 and 1000 nM. 9. Rinse the cells three times with PBS+.10. Fix the cells for 10 min in 3.7% formaldehyde in PBS (pH 7.4). 11. Aspirate and rinse the cells three times for 5 min each in PBS. 12. Mount the coverslips. 13. Image the cells.
MitoTracker Orange CMTMRos is a fluorescent dye that labels mitochondria within live cells utilizing the mitochondrial membrane potential (Ex/Em: 551/576 nm).
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Cells: For live cells, follow Steps 1–7. For fixed cells, follow Steps 8–13. 1. Prepare a labeling solution at 25–500 nM in the desired cell culture medium, and warm it to the cell culture temperature. 2. Aspirate the culture medium from cells grown on coverslips. 3. Immerse the cells in the labeling medium for 15–45 min at 37?C. 4. Aspirate the labeling medium, and rinse the cells three times with culture medium. 5. Mount the cells. 6. Check the cells using a fluorescence microscope. If the fluorescence staining is too low, try one of the following options: i. Incubate for an additional 30 min in the normal culture medium to allow the thiol conjugation to proceed. ii. Try a higher initial concentration of the labeling solution. 7. When the fluorescence is sufficient, image the cells. 8. Carry out Steps 1–4, but use a labeling solution at a working concentration between 100 and 1000 nM. 9. Rinse the cells three times with PBS+.10. Fix the cells for 10 min in 3.7% formaldehyde in PBS (pH 7.4). 11. Aspirate and rinse the cells three times for 5 min each in PBS. 12. Mount the coverslips. 13. Image the cells.
产品资料
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