产品
编 号：F743525
分子式：C16H15N3
分子量：249.31
分子式：C16H15N3
分子量：249.31
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CAS No: 125113-96-4
产品详情
生物活性：
DCDAPH (Compound 2c) is a novel smart NIRF probe for detection of β-amyloid (Aβ) plaques (λex/λem=597/665 nm in PBS). DCDAPH shows high affinity for Aβ aggregates (Ki=37 nM, Kd=27 nM). DCDAPH shows good blood brain barrier permeation and can meet most of the requirements for the detection of Aβ plaques both in vitro and in vivo.
体内研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Fluorescent Staining of DCDAPH to Aβ Plaques in Mouse Brain.1. Mouse should be i.v. injected with DCDAPH (0.4 mg/kg, 20% DMSO (HY-Y0320), 80% propylene glycol (HY-Y0921), 50 μL), and sacrificed at 30 min after injection.2. The brains should be excised, embedded in optimum cutting temperature compound (OCT), and frozen in powdered dry ice immediately.3. Frozen sections of 20 μm should be cut.4. Fluorescent observation (Cy5 filter set).
体外研究：
DCDAPH (0-10 μM; 24 h) treatment shows no toxicity to human neuronal cells.
DCDAPH (Compound 2c) is a novel smart NIRF probe for detection of β-amyloid (Aβ) plaques (λex/λem=597/665 nm in PBS). DCDAPH shows high affinity for Aβ aggregates (Ki=37 nM, Kd=27 nM). DCDAPH shows good blood brain barrier permeation and can meet most of the requirements for the detection of Aβ plaques both in vitro and in vivo.
体内研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Fluorescent Staining of DCDAPH to Aβ Plaques in Mouse Brain.1. Mouse should be i.v. injected with DCDAPH (0.4 mg/kg, 20% DMSO (HY-Y0320), 80% propylene glycol (HY-Y0921), 50 μL), and sacrificed at 30 min after injection.2. The brains should be excised, embedded in optimum cutting temperature compound (OCT), and frozen in powdered dry ice immediately.3. Frozen sections of 20 μm should be cut.4. Fluorescent observation (Cy5 filter set).
体外研究：
DCDAPH (0-10 μM; 24 h) treatment shows no toxicity to human neuronal cells.
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