产品
编 号：F742905
分子式：C19H20BF2N3O
分子量：355.19
分子式：C19H20BF2N3O
分子量：355.19
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规格
价格
是否有货
结构图
CAS No: 1181844-41-6
产品详情
生物活性：
o-Pah is a BODIPY derivative with an -NH2 and -OH substituted meso-Ph group. o-Pah exhibits metal-induced J-aggregation in the presence of Cu2+ and a specific fluorescence enhancement for Hg2+ (Ex/Em=483/(495-600) nM).
体外研究：
o-Pah (20 μM) shows changed colour upon addition of 100 μM different metal ions and the further addition of 20 μM of Cu2+ in DMSO/HEPES buffer solution.Procedures of metal ion sensing Stock solutions of the metal ions (100 mM and 10 mM) were prepared in deionised water.1. A stock solution of o-Pah (0.5 mM) is prepared in DMSO.2. The solution of o-Pah is then diluted to 5 μM with HEPES buffer solution (50 mM KNO3, 50 mM HEPRS, pH 7.2).3. During titration experiments, 2 mL solutions of o-Pah (5 μM, 0.5 μM) are placed in a 1 cm quartz optical cell and Cu2+, Hg2+ stock solutions are added gradually with a micropipette.4. In selectivity experiments, the test samples are prepared by adding appropriate amounts of the metal ion stock solution into the 2 mL solution of o-Pah (5 μM).6. During fluorescence measurements, the excitation wavelength is 483 nm and emission spectra are collected between 495-600 nm.
o-Pah is a BODIPY derivative with an -NH2 and -OH substituted meso-Ph group. o-Pah exhibits metal-induced J-aggregation in the presence of Cu2+ and a specific fluorescence enhancement for Hg2+ (Ex/Em=483/(495-600) nM).
体外研究：
o-Pah (20 μM) shows changed colour upon addition of 100 μM different metal ions and the further addition of 20 μM of Cu2+ in DMSO/HEPES buffer solution.Procedures of metal ion sensing Stock solutions of the metal ions (100 mM and 10 mM) were prepared in deionised water.1. A stock solution of o-Pah (0.5 mM) is prepared in DMSO.2. The solution of o-Pah is then diluted to 5 μM with HEPES buffer solution (50 mM KNO3, 50 mM HEPRS, pH 7.2).3. During titration experiments, 2 mL solutions of o-Pah (5 μM, 0.5 μM) are placed in a 1 cm quartz optical cell and Cu2+, Hg2+ stock solutions are added gradually with a micropipette.4. In selectivity experiments, the test samples are prepared by adding appropriate amounts of the metal ion stock solution into the 2 mL solution of o-Pah (5 μM).6. During fluorescence measurements, the excitation wavelength is 483 nm and emission spectra are collected between 495-600 nm.
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