产品
编 号：F741647
分子式：C21H22N4
分子量：330.43
分子式：C21H22N4
分子量：330.43
产品类型
规格
价格
是否有货
10mM*1mL in DMSO
询价
询价
1mg
询价
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5mg
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10mg
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询价
结构图
CAS No: 2530161-67-0
产品详情
生物活性：
HBC514 is a nonfluorescent HBC-analog but emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. HBC514-Pepper complex enables visualization of RNAs and the fluorescences can be altered flexibly by simple washing and staining in living Pepper-tagged cells.
体外研究：
HBC is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor, nonfluorescent in solution. ‘Pepper’ is able to enhance HBC’s fluorescence more than 3,000-fold and exhibits bright fluorescence when expressed in living cells.‘Pepper’ has reduced Mg2+ sensitivity after multiple rounds of optimization, displays a well-defined fluorescent profile with an excitation maximum at 485nm, an emission maximum at 530nm, and excellent brightness.HBC514 (2-10 μM) is altered flexibly by simple washing and staining by Pepper based CRISPR imaging in live HeLa cells (expressing sgRNA-Pep1 and dCas9).
HBC514 is a nonfluorescent HBC-analog but emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. HBC514-Pepper complex enables visualization of RNAs and the fluorescences can be altered flexibly by simple washing and staining in living Pepper-tagged cells.
体外研究：
HBC is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor, nonfluorescent in solution. ‘Pepper’ is able to enhance HBC’s fluorescence more than 3,000-fold and exhibits bright fluorescence when expressed in living cells.‘Pepper’ has reduced Mg2+ sensitivity after multiple rounds of optimization, displays a well-defined fluorescent profile with an excitation maximum at 485nm, an emission maximum at 530nm, and excellent brightness.HBC514 (2-10 μM) is altered flexibly by simple washing and staining by Pepper based CRISPR imaging in live HeLa cells (expressing sgRNA-Pep1 and dCas9).
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