产品
编 号：F704954
分子式：C59H62N6O21S
分子量：1223.22
分子式：C59H62N6O21S
分子量：1223.22
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规格
价格
是否有货
结构图
CAS No: 172646-19-4
产品详情
生物活性：
Calcium Orange AM is an intracellular calcium reporter. Specific fluorescence can be detected when free calcium binds to Calcium Orange AM (Ex/Em=549/576 nm). Calcium Orange AM does not enter the vacuoles and does not compartmentalize into acidic vesicles.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Calcium Orange AM is freshly prepared in dehydrated DMSO before each experiment. 2. Prepare the recording medium for measuring [Ca2+]i in cells consisted of 20 mM HEPES, 115 mM NaCl, 5.4 mM KCl, 0.8 mM MgCl2, 1.8 mM CaCl2, and 13.8 mM D-glucose adjusted to pH 7.4 by adding 1 M KOH. 3. Exchange the culture medium for 1 mL of a recording medium including 10 μM Calcium Orange AM. 4. Incubate cells under conditions of 5% CO2 and 37°C for 40 min. 5. Remove the recording medium, including calcium orange AM from the dish, and add the culture medium after washing it three times with 1 mL of the recording medium. 6. Incubate the cells for 1 h. After incubation, exchange the culture medium for the recording medium again. 7. Detect fluorescence.
Calcium Orange AM is an intracellular calcium reporter. Specific fluorescence can be detected when free calcium binds to Calcium Orange AM (Ex/Em=549/576 nm). Calcium Orange AM does not enter the vacuoles and does not compartmentalize into acidic vesicles.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Calcium Orange AM is freshly prepared in dehydrated DMSO before each experiment. 2. Prepare the recording medium for measuring [Ca2+]i in cells consisted of 20 mM HEPES, 115 mM NaCl, 5.4 mM KCl, 0.8 mM MgCl2, 1.8 mM CaCl2, and 13.8 mM D-glucose adjusted to pH 7.4 by adding 1 M KOH. 3. Exchange the culture medium for 1 mL of a recording medium including 10 μM Calcium Orange AM. 4. Incubate cells under conditions of 5% CO2 and 37°C for 40 min. 5. Remove the recording medium, including calcium orange AM from the dish, and add the culture medium after washing it three times with 1 mL of the recording medium. 6. Incubate the cells for 1 h. After incubation, exchange the culture medium for the recording medium again. 7. Detect fluorescence.
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