产品
编 号：F622241
分子式：C13H15NO2
分子量：217.26
分子式：C13H15NO2
分子量：217.26
产品类型
规格
价格
是否有货
10mM*1mL in DMSO
询价
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5mg
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10mg
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25mg
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50mg
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100mg
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结构图
CAS No: 1694495-59-4
产品详情
生物活性：
Alkyne-phenol (Alk-Ph) is a clickable ascorbate peroxidase 2 (APEX2) probe. Alkyne-phenol substantially improves APEX-labeling efficiency in intact yeast cells, as it is more cell wall-permeant than APEX2 substrate biotin-phenol (BP). Alkyne-phenol also facilitates the identification of APEX-labeling sites, allowing the unambiguous assignment of membrane topology of mitochondrial proteins. Alkyne-phenol is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
体外研究：
将表达 Su9-APEX2 的酵母细胞与每个探针以 2.5 mM 的最终浓度孵育半小时，并通过添加 1 mM H2O2 启动标记反应。Alkyne-phenol 是标记酵母蛋白质组的最具活性的 APEX2 底物。酵母线粒体中 APEX2 介导的 Alkyne-phenol 标记在探针浓度高于 1 mM 时更有效。Alkyne-phenol 能够以极高的特异性 (94%) 对完整酵母细胞的线粒体基质进行蛋白质组学分析，并提供比传统 APEX2 底物生物素-苯酚 (BP) 更高的覆盖率。
Alkyne-phenol (Alk-Ph) is a clickable ascorbate peroxidase 2 (APEX2) probe. Alkyne-phenol substantially improves APEX-labeling efficiency in intact yeast cells, as it is more cell wall-permeant than APEX2 substrate biotin-phenol (BP). Alkyne-phenol also facilitates the identification of APEX-labeling sites, allowing the unambiguous assignment of membrane topology of mitochondrial proteins. Alkyne-phenol is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
体外研究：
将表达 Su9-APEX2 的酵母细胞与每个探针以 2.5 mM 的最终浓度孵育半小时，并通过添加 1 mM H2O2 启动标记反应。Alkyne-phenol 是标记酵母蛋白质组的最具活性的 APEX2 底物。酵母线粒体中 APEX2 介导的 Alkyne-phenol 标记在探针浓度高于 1 mM 时更有效。Alkyne-phenol 能够以极高的特异性 (94%) 对完整酵母细胞的线粒体基质进行蛋白质组学分析，并提供比传统 APEX2 底物生物素-苯酚 (BP) 更高的覆盖率。
产品资料
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