产品
编 号：F065029
分子式：C14H11NO4
分子量：257.24
分子式：C14H11NO4
分子量：257.24
产品类型
规格
价格
是否有货
1mg
240
In-stock
5mg
680
In-stock
10mg
1055
In-stock
结构图
CAS No: 119171-73-2
产品详情
生物活性：
ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).
体外研究：
To obtain the parameters Km and kcat for Compound I,two independent methods are used. Initially, the oxidation of ADHP using the injector functionality built-in to the fluorescence plate reader is studied. The auto-injector dispenses the H2O2 to initiate the reaction, as a means of generating a set of progress curves. Analysis for MPO-mediated oxidation of ADHP gives a Km of 31±4 μM and the kcat of 186± 6 s 1.The kobs also increases over the experimental range of ADHP concentrations from 1 to 80 μM and for the converse experiment holding substrate constant over 3 to 45 nM MPO. The apparent second order rate constant obtain from the slope of kobs against ADHP concentration Kappon is 2.1±0.2 mM/s.
ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).
体外研究：
To obtain the parameters Km and kcat for Compound I,two independent methods are used. Initially, the oxidation of ADHP using the injector functionality built-in to the fluorescence plate reader is studied. The auto-injector dispenses the H2O2 to initiate the reaction, as a means of generating a set of progress curves. Analysis for MPO-mediated oxidation of ADHP gives a Km of 31±4 μM and the kcat of 186± 6 s 1.The kobs also increases over the experimental range of ADHP concentrations from 1 to 80 μM and for the converse experiment holding substrate constant over 3 to 45 nM MPO. The apparent second order rate constant obtain from the slope of kobs against ADHP concentration Kappon is 2.1±0.2 mM/s.
产品资料
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