产品
编 号：F597430
分子式：C28H18F2K5N3O14
分子量：853.94
分子式：C28H18F2K5N3O14
分子量：853.94
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规格
价格
是否有货
结构图
CAS No: 192140-58-2
产品详情
生物活性：
Fura-FF pentapotassium is a low-affinity fluorescent dye for calcium (Ex/Em: 365/514 nm in the absence of calcium; 339/507 nm in the presence of a high calcium concentration).
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Human Sperm Cells: 1. Incubate the cells according to your normal protocol. 2. Load cells with 5 μM Fura-FF in 1 mL Hepes-buffered human sperm (H-HSM) medium at 36 ℃ for 40 min. 3. Wash the sample by centrifugation and scatter cells in disposable centrifuge tubes at 1 107 cells in 3 mL of H-HSM and maintained at 36 ℃. 4. Immediately measure fluorescence recordings.
Fura-FF pentapotassium is a low-affinity fluorescent dye for calcium (Ex/Em: 365/514 nm in the absence of calcium; 339/507 nm in the presence of a high calcium concentration).
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Human Sperm Cells: 1. Incubate the cells according to your normal protocol. 2. Load cells with 5 μM Fura-FF in 1 mL Hepes-buffered human sperm (H-HSM) medium at 36 ℃ for 40 min. 3. Wash the sample by centrifugation and scatter cells in disposable centrifuge tubes at 1 107 cells in 3 mL of H-HSM and maintained at 36 ℃. 4. Immediately measure fluorescence recordings.
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