产品
编 号：F578134
分子式：C30H50BF2N3S4
分子量：629.81
分子式：C30H50BF2N3S4
分子量：629.81
产品类型
规格
价格
是否有货
1mg
询价
询价
5mg
询价
询价
10mg
询价
询价
结构图
CAS No: 874748-20-6
产品详情
生物活性：
Coppersensor-1 (CS1) is a membrane-permeable fluorescent dye. Coppersensor-1 has a picomolar affinity for Cu+ with high selectivity over competing cellular metalions. Coppersensor-1 as a probe, can selective and sensitive detection of copper(I) ions (Cu+) in biological samples, including live cells. Coppersensor-1 can be used for the research of imaging of severe diseases such as cancer, cardiovascular disorders and neurogenerative diseases.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).A. Preparation of reagent stock solutions for imaging: 1. Prepare a 1 mM stock solution of CS1 (MW 630 g/moL) in DMSO by dissolving 0.63 mg of solid CS1 per milliliter of DMSO solvent.2. Prepare a 10 mM stock solution of CuCl or CuCl2.B. CS1 labeling of live cells: 1. Imaging experiments with cells will require 1-2 d with cells in culture. Incubate the cells according to your normal protocol.2. One day before imaging, cells are passaged and plated on 18-mm glass coverslips coated with poly-L-lysine (50 μg/mL). Adherentcells for imaging are grown to 50-80% confluency.3. Remove cells from incubator, and transfer one coverslip into a 35-mm Petri dish containing 3 mL PBS buffer. 4. To the Petri dish, add 15 μL of a 1 mM CS1 stock solution to give a final dye concentration of 5 μM. Mix thoroughly.(Higher concentrations of dye may result in high levels of background fluorescence)5. Incubate for 5-20 min in the dark at 25 or 37℃.C. Imaging the CS1-labeled cells:1. Imageing in standard confocal microscope with 543 nm excitation.(CS1 can be imaged using any type of fluorescence microscope, including epifluorescence, confocal and multiphoton)
Coppersensor-1 (CS1) is a membrane-permeable fluorescent dye. Coppersensor-1 has a picomolar affinity for Cu+ with high selectivity over competing cellular metalions. Coppersensor-1 as a probe, can selective and sensitive detection of copper(I) ions (Cu+) in biological samples, including live cells. Coppersensor-1 can be used for the research of imaging of severe diseases such as cancer, cardiovascular disorders and neurogenerative diseases.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).A. Preparation of reagent stock solutions for imaging: 1. Prepare a 1 mM stock solution of CS1 (MW 630 g/moL) in DMSO by dissolving 0.63 mg of solid CS1 per milliliter of DMSO solvent.2. Prepare a 10 mM stock solution of CuCl or CuCl2.B. CS1 labeling of live cells: 1. Imaging experiments with cells will require 1-2 d with cells in culture. Incubate the cells according to your normal protocol.2. One day before imaging, cells are passaged and plated on 18-mm glass coverslips coated with poly-L-lysine (50 μg/mL). Adherentcells for imaging are grown to 50-80% confluency.3. Remove cells from incubator, and transfer one coverslip into a 35-mm Petri dish containing 3 mL PBS buffer. 4. To the Petri dish, add 15 μL of a 1 mM CS1 stock solution to give a final dye concentration of 5 μM. Mix thoroughly.(Higher concentrations of dye may result in high levels of background fluorescence)5. Incubate for 5-20 min in the dark at 25 or 37℃.C. Imaging the CS1-labeled cells:1. Imageing in standard confocal microscope with 543 nm excitation.(CS1 can be imaged using any type of fluorescence microscope, including epifluorescence, confocal and multiphoton)
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