产品
编 号：F479940
分子式：C54H40O22
分子量：520.44
分子式：C54H40O22
分子量：520.44
产品类型
规格
价格
是否有货
1mg
询价
询价
5mg
询价
询价
10mg
询价
询价
结构图
CAS No: 85138-49-4
产品详情
生物活性：
BCECF is a pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0–8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Prepare a 2-20 mM stock solution of BCECF in DMSO and store at -20℃. 2. Prepare a 5-50 μM BCECF dye-loading solution in buffer solutions (Hepes, pH 7.4or PBS).3. Add 1000 μL/well (6-well plate),100 μL/well (96-well plate) or 25 μL/well (384-well plate) BCECF dye-loading solution into the cell plate.4. Incubate the dye-loading plate in a cell incubator about 30minutes.5. Wash and replace the dye-loading solution with buffers.6. Run the pH assay by monitoring the fluorescence at Ex/Em = 490/535 nm or 430/535 nm for ratio measurements.
BCECF is a pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0–8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Prepare a 2-20 mM stock solution of BCECF in DMSO and store at -20℃. 2. Prepare a 5-50 μM BCECF dye-loading solution in buffer solutions (Hepes, pH 7.4or PBS).3. Add 1000 μL/well (6-well plate),100 μL/well (96-well plate) or 25 μL/well (384-well plate) BCECF dye-loading solution into the cell plate.4. Incubate the dye-loading plate in a cell incubator about 30minutes.5. Wash and replace the dye-loading solution with buffers.6. Run the pH assay by monitoring the fluorescence at Ex/Em = 490/535 nm or 430/535 nm for ratio measurements.
产品资料
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