产品
编 号：F055619
分子式：C40H36O19
分子量：820.7
分子式：C40H36O19
分子量：820.7
产品类型
规格
价格
是否有货
1mg
询价
询价
结构图
CAS No: 117464-70-7
产品详情
生物活性：
BCECF-AM is a cell membrane permeable compound widely used as a fluorescent indicator for intracellular pH.
体外研究：
Fully treated cells show hydrogenosomes with an electron-dense deposit which aggregates to a variable extent.The staining is seen in the interior ofhydrogenosomes in some instances. It is also observed by microscopy that the K+/H+ ionophor nigericin does not inhibit hydrogenosomal loading with BCECF-AM.The pH-sensitive fluorescent dyes to measure cytosolic pH.1.Prepare a 2 to 20 mM stock solution of BCECF-AM in DMSO. 2.Prepare a 5-50 μM BCECF-AM dye-loading solution inbuffer solutions (HHBS or PBS).3. Add 1000 μL/well (6-well plate),100 μL/well (96-well plate) or 25 μL/well (384-well plate) BCECF-AM dye-loading solution into the cell plate.4. Incubate the dye-loading plate in a cell incubator for 30-60 minutes.5. Wash and replace the dye-loading solution with buffers.6. Run the pH assay by monitoring the fluorescence at Ex/Em = 490/535 nm or 430/535 nm for ratio measurements.
BCECF-AM is a cell membrane permeable compound widely used as a fluorescent indicator for intracellular pH.
体外研究：
Fully treated cells show hydrogenosomes with an electron-dense deposit which aggregates to a variable extent.The staining is seen in the interior ofhydrogenosomes in some instances. It is also observed by microscopy that the K+/H+ ionophor nigericin does not inhibit hydrogenosomal loading with BCECF-AM.The pH-sensitive fluorescent dyes to measure cytosolic pH.1.Prepare a 2 to 20 mM stock solution of BCECF-AM in DMSO. 2.Prepare a 5-50 μM BCECF-AM dye-loading solution inbuffer solutions (HHBS or PBS).3. Add 1000 μL/well (6-well plate),100 μL/well (96-well plate) or 25 μL/well (384-well plate) BCECF-AM dye-loading solution into the cell plate.4. Incubate the dye-loading plate in a cell incubator for 30-60 minutes.5. Wash and replace the dye-loading solution with buffers.6. Run the pH assay by monitoring the fluorescence at Ex/Em = 490/535 nm or 430/535 nm for ratio measurements.
产品资料
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