产品
编 号:F470996
分子式:C12H18NNaO4S
分子量:295.33
产品类型
结构图
CAS No: 82692-93-1
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产品详情
生物活性:
TOOS (TOOS sodium salt) is a highly water-soluble aniline derivative widely used in diagnostics and biological experiments. TOOS can be combined with 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) to form a chromogenic system to measure oxidase activity. In the MBTH-TOOS chromogenic system, MBTH is catalytically oxidized to produce (-NH) free radicals, which react with TOOS to form colorless compounds. Furthermore, the colorless compound undergoes a disproportionation reaction to produce a blue-violet quinoid compound.
体外研究:
MBTH-TOOS 显色系统以 Cit-PtNPs 氧化酶为例:将 50 μL Cit-PtNP、250 μL 3 mM TOOS 和 200 μL 0.5 mM MBTH 混合到 500 μL Tris-HCl 缓冲溶液 (10 mM,pH=8.0) 中,并在 40°C 下孵育 25 分钟。随后,在 590 nm 处测定吸光度,计算氧化酶活性水平。