产品
编 号：F410322
分子式：C26H32N3NaO6S2
分子量：569.67
分子式：C26H32N3NaO6S2
分子量：569.67
产品类型
规格
价格
是否有货
5mg
询价
询价
10mg
询价
询价
结构图
CAS No: 62796-23-0
产品详情
生物活性：
Merocyanin 540 is a fluorescent membrane probe that selectively stains the membranes of a wide variety of electrically excitable cells, but not those of nonexcitable cells (Ex/Em: 540/580 nm).
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Cells: 1. Wash monolayer cultured cells with isotonic saline with or without 1-5 mM CaCl2 or iso-osmotic sucrose (0.25 M) solutions buffered with 5 mM Tris-Cl (pH 7.4). 2. Expose cells to Merocyanin 540 (1.6 x 10-5 M) in the appropriate buffer for 10 min at room temperature under ambient lighting conditions. 3. Wash extensively and examine by fluorescence microscopy. 4. For protozoa, place 0.05 mL aliquots of washed cultures containing 100-200 organisms on a microscope slide. 5. Add Merocyanin 540 to a final concentration of 1.0-10 μg/mL. 6. Examine cells by fluorescence microscopy. 7. Incubate single cell suspensions or tissue slices with Merocyanin 540 (1.75-5.25 x 10-5 M) and examine by fluorescence microscopy as described for attached cells.
Merocyanin 540 is a fluorescent membrane probe that selectively stains the membranes of a wide variety of electrically excitable cells, but not those of nonexcitable cells (Ex/Em: 540/580 nm).
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Cells: 1. Wash monolayer cultured cells with isotonic saline with or without 1-5 mM CaCl2 or iso-osmotic sucrose (0.25 M) solutions buffered with 5 mM Tris-Cl (pH 7.4). 2. Expose cells to Merocyanin 540 (1.6 x 10-5 M) in the appropriate buffer for 10 min at room temperature under ambient lighting conditions. 3. Wash extensively and examine by fluorescence microscopy. 4. For protozoa, place 0.05 mL aliquots of washed cultures containing 100-200 organisms on a microscope slide. 5. Add Merocyanin 540 to a final concentration of 1.0-10 μg/mL. 6. Examine cells by fluorescence microscopy. 7. Incubate single cell suspensions or tissue slices with Merocyanin 540 (1.75-5.25 x 10-5 M) and examine by fluorescence microscopy as described for attached cells.
产品资料
Copyright©2015-2024 沪ICP备2022026524号-1
沪公网安备