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编 号：F397923
分子式：C25H25N2Na2O7S2+
分子量：575.58
分子式：C25H25N2Na2O7S2+
分子量：575.58
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CAS No: 5873-16-5
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生物活性：
Sulforhodamine G is a fluorescent stain with broad dynamic ranges. Sulforhodamine G can be used for the research of protein stains.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Labeling of fluorescent internal protein:A. Prepared of protein samples: 1. Protein samples to be analyzed are spiked with 0.1% of the total protein load of ALIS647 (ALIS internal standard) prior.2. Protein sample is separated with 2-DE in the dark.B. Purify Sulforhodamine G:1. Sulforhodamine G(60% purity, 10 mg) dissolved in 100 mL of 1% v/v acetic acid to purify by RP chromatography.2. Collect the pool with an absorbance maximum at 528 nm, lyophilized to dryness and stored as a dry powder at 47℃.C. Stainning:1. staining was performed in polypropylene staining disheswrapped in aluminum foil to prevent photobleaching of thestains.2. Sulforhodamine G staining is performed overnight in35% methanol with a four-fold molar excess of dye: proteinbased on an average protein molecular weight of 50 kDa.3. Following 4×15 min washes in 35% methanol and2×15 min equilibrations in water.4. Total protein and ALIS are visualized with a laser scanner using differentchannels.5. Protein spots visualized using total protein stain (λex=532 nm) and ALIS (λex=633 nm) are quantified separately using 2-DE software, and statistical analysis.
Sulforhodamine G is a fluorescent stain with broad dynamic ranges. Sulforhodamine G can be used for the research of protein stains.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Labeling of fluorescent internal protein:A. Prepared of protein samples: 1. Protein samples to be analyzed are spiked with 0.1% of the total protein load of ALIS647 (ALIS internal standard) prior.2. Protein sample is separated with 2-DE in the dark.B. Purify Sulforhodamine G:1. Sulforhodamine G(60% purity, 10 mg) dissolved in 100 mL of 1% v/v acetic acid to purify by RP chromatography.2. Collect the pool with an absorbance maximum at 528 nm, lyophilized to dryness and stored as a dry powder at 47℃.C. Stainning:1. staining was performed in polypropylene staining disheswrapped in aluminum foil to prevent photobleaching of thestains.2. Sulforhodamine G staining is performed overnight in35% methanol with a four-fold molar excess of dye: proteinbased on an average protein molecular weight of 50 kDa.3. Following 4×15 min washes in 35% methanol and2×15 min equilibrations in water.4. Total protein and ALIS are visualized with a laser scanner using differentchannels.5. Protein spots visualized using total protein stain (λex=532 nm) and ALIS (λex=633 nm) are quantified separately using 2-DE software, and statistical analysis.
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