产品
编 号：F375070
产品类型
规格
价格
是否有货
500mg
询价
询价
1g
询价
询价
5g
询价
询价
结构图
CAS No: 51395-97-2
产品详情
生物活性：
Mucicarmine is used in the histological visualization of acid mucopolysaccharides in tissue sections. Mucicarmine can identify mucin (deep rose), which is useful in determining the mucin positive cancer (such as liver carcinoma).
体外研究：
Mucicarmine successfully stains the presence of mucin on frozen-tissue sections (Mucin stained deep rose).Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Mount the cryostat sections (thickness: 4 to 5 μm) onto coated glass slides.2. Fix the sections in frozen-section fixative immediately for 30 seconds and then rinse with water for 5 seconds.3. Immerse the glass slides for 30 seconds into Harris hematoxylin, then consecutively into distilled water, ammonium hydroxide, and distilled water, for 5 seconds in each solution.4. The mucicarmine stain is placed in the microwave oven and microwave until the solution is about to bubble (30 seconds).5. Immerse the glass slides in the warmed mucicarmine solution for 2 minutes and rinse slides in distilled water for 5 seconds.7. Immerse the glass slides consecutively in 95% alcohol, 100% alcohol, and xylene for 5 seconds in each solution, then coverslip the slides.Note: Nuclei stain (blue) and connective tissue stain (green) maybe is necessary according to your specific needs.
Mucicarmine is used in the histological visualization of acid mucopolysaccharides in tissue sections. Mucicarmine can identify mucin (deep rose), which is useful in determining the mucin positive cancer (such as liver carcinoma).
体外研究：
Mucicarmine successfully stains the presence of mucin on frozen-tissue sections (Mucin stained deep rose).Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Mount the cryostat sections (thickness: 4 to 5 μm) onto coated glass slides.2. Fix the sections in frozen-section fixative immediately for 30 seconds and then rinse with water for 5 seconds.3. Immerse the glass slides for 30 seconds into Harris hematoxylin, then consecutively into distilled water, ammonium hydroxide, and distilled water, for 5 seconds in each solution.4. The mucicarmine stain is placed in the microwave oven and microwave until the solution is about to bubble (30 seconds).5. Immerse the glass slides in the warmed mucicarmine solution for 2 minutes and rinse slides in distilled water for 5 seconds.7. Immerse the glass slides consecutively in 95% alcohol, 100% alcohol, and xylene for 5 seconds in each solution, then coverslip the slides.Note: Nuclei stain (blue) and connective tissue stain (green) maybe is necessary according to your specific needs.
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