产品
编 号：F331498
分子式：C14H19N3O5S
分子量：341.38
分子式：C14H19N3O5S
分子量：341.38
产品类型
规格
价格
是否有货
100mg
480
In-stock
200mg
760
In-stock
结构图
CAS No: 35013-72-0
产品详情
生物活性：
Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).?Calculations:?For best results, use ≥ 12-fold molar excess of biotin for a 10 mg/mL protein solution or ≥ 20-fold molar excess of biotin for a 2 mg/mL protein solution.?1.Calculate millimoles of biotin reagent to add to the reaction for a 20-fold molar excess:?mL protein × (mg protein/mL protein) × (mmoL protein/mg protein) × (20 mmoL protein/mmoL protein) = mmoL Biotin.?* 20 = Recommended molar fold excess of biotin for 2 mg/ml protein sample?2. Calculate microliters of 10 mM biotin reagent solution (prepared in Step B.3) to add to the reaction: ?mmoL Biotin × (1000000 μL/L) × (L/ 10 mmoL) =μL Biotin?Biotin Labeling Reaction:?1. Dissolve 1-10 mg protein in 0.5-2.0 mL PBS according to the calculation made in Calculations. ?2. Before use, prepare a 10 mM solution of the biotin reagent in an organic solvent such as DMSO. ?3. Add the appropriate volume (μL) of 10 mM biotin reagent solution to the protein solution. ?4. Incubate reaction on ice for two hours or at room temperature for 30 minutes. ?5. To tests the labeled protein by ELISA or Western blot.
Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).?Calculations:?For best results, use ≥ 12-fold molar excess of biotin for a 10 mg/mL protein solution or ≥ 20-fold molar excess of biotin for a 2 mg/mL protein solution.?1.Calculate millimoles of biotin reagent to add to the reaction for a 20-fold molar excess:?mL protein × (mg protein/mL protein) × (mmoL protein/mg protein) × (20 mmoL protein/mmoL protein) = mmoL Biotin.?* 20 = Recommended molar fold excess of biotin for 2 mg/ml protein sample?2. Calculate microliters of 10 mM biotin reagent solution (prepared in Step B.3) to add to the reaction: ?mmoL Biotin × (1000000 μL/L) × (L/ 10 mmoL) =μL Biotin?Biotin Labeling Reaction:?1. Dissolve 1-10 mg protein in 0.5-2.0 mL PBS according to the calculation made in Calculations. ?2. Before use, prepare a 10 mM solution of the biotin reagent in an organic solvent such as DMSO. ?3. Add the appropriate volume (μL) of 10 mM biotin reagent solution to the protein solution. ?4. Incubate reaction on ice for two hours or at room temperature for 30 minutes. ?5. To tests the labeled protein by ELISA or Western blot.
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