产品
编 号：F309980
分子式：C18H16BrN5S
分子量：414.32
分子式：C18H16BrN5S
分子量：414.32
产品类型
规格
价格
是否有货
500mg
400
In-stock
1g
640
In-stock
5g
1720
In-stock
结构图
CAS No: 298-93-1
产品详情
生物活性：
Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.
体外研究：
1.MTT 工作液的配制用 PBS 溶解 MTT，得到5 mg/mL的MTT。 2.细胞增殖检测 (96孔板)2.1 接种细胞：制备的单细胞用含10%FBS的培养基制备悬浮液，接种于96孔板，每孔1000-10000个细胞，每孔体积 100 μL。2.2培养细胞：37℃。5% CO2，孵育24-72 h。2.3每孔加入10 μL MTT，孵育4 h，弃上清，悬浮细胞需先离心。2.4 加入100 μL DMSO，震荡10分钟使晶体完全溶解 2.5 用吸光度板读数仪在OD=562 nm处监测吸光度的增加。
Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.
体外研究：
1.MTT 工作液的配制用 PBS 溶解 MTT，得到5 mg/mL的MTT。 2.细胞增殖检测 (96孔板)2.1 接种细胞：制备的单细胞用含10%FBS的培养基制备悬浮液，接种于96孔板，每孔1000-10000个细胞，每孔体积 100 μL。2.2培养细胞：37℃。5% CO2，孵育24-72 h。2.3每孔加入10 μL MTT，孵育4 h，弃上清，悬浮细胞需先离心。2.4 加入100 μL DMSO，震荡10分钟使晶体完全溶解 2.5 用吸光度板读数仪在OD=562 nm处监测吸光度的增加。
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