产品
编 号：F032347
分子式：C44H47N3O24
分子量：1001.85
分子式：C44H47N3O24
分子量：1001.85
产品类型
规格
价格
是否有货
100μg
询价
询价
1mg
询价
询价
结构图
CAS No: 108964-32-5
产品详情
生物活性：
Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca2+ indicator.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Fura-2 AM diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid.1.First, prepare the 1 mM Fura-2 AM stock by adding 50 μL of DMSO to a 50 μg vial. It is important to use dry DMSO packed under nitrogen and it is necessary to remove the DMSO with a needle by puncturing the septum to prevent hydration of the DMSO. After preparing the Fura-2 AM solution keep it in a dark dry place. Fura-2 AM in DMSO is stable at RT for 24 hours and is stable at -20 degrees in a dry container for several months.2.Aliquot 2 mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 μL of Fura-2 AM stock to generate a 1μM Fura-2 AM solution. Vortex the solution vigorously for 1 min.3.Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish.4.Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Time the incubation precisely.5.Prepare a 35 mm dish containing 2 mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish.6.Mount the coverslip on the imaging chamber.
Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca2+ indicator.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Fura-2 AM diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid.1.First, prepare the 1 mM Fura-2 AM stock by adding 50 μL of DMSO to a 50 μg vial. It is important to use dry DMSO packed under nitrogen and it is necessary to remove the DMSO with a needle by puncturing the septum to prevent hydration of the DMSO. After preparing the Fura-2 AM solution keep it in a dark dry place. Fura-2 AM in DMSO is stable at RT for 24 hours and is stable at -20 degrees in a dry container for several months.2.Aliquot 2 mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 μL of Fura-2 AM stock to generate a 1μM Fura-2 AM solution. Vortex the solution vigorously for 1 min.3.Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish.4.Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Time the incubation precisely.5.Prepare a 35 mm dish containing 2 mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish.6.Mount the coverslip on the imaging chamber.
产品资料
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