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编 号:F276711
分子式:C32H43N5O11
分子量:673.71
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生物活性:
Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm).

体外研究:
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Enzyme activity (rabbit osteoclasts for example):1. Treat purified, differentiated cells by medium for 48 h (time depends on the cell).2. Put treated cells into 4.5 mL lysis buffer.3. Each lysate is divided into thirds, then incubate with 40 μM Ac-VEID-AMC at 37°C for 1 h.4. Detect cleavage of fluorescent substrates (Ex=340-360 nm, Em=440-460 nm)
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