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编 号：F275608
分子式：C20H26N4O11P2
分子量：560.39
分子式：C20H26N4O11P2
分子量：560.39
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CAS No: 217305-49-2
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生物活性：
Fluorescein diphosphate tetraammonium is a fluorescent dye, it can be used as a fluorogenic substrate and generats fluorescein as a fluorescent product.
体外研究：
Fluorescein diphosphate tetraammonium can be used as a fluorogenic substrate, and obtains luorescein as a fluorescent product.Fluorescein diphosphate tetraammonium (50 μM; room temperature) can be used as a substrate in vitro phosphatase assays.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).ELISA with capillary immunosensor (single-step ELISA):1. Put various concentrations of antigen in Tris buffer 1 containing 1% BSA into capillary immunosensor.2. Immobilize capture antibody and enzyme-linked antibody via capillary action, incubates for a preset time, and subsequently washing with 1 mL of 50 mM Tris buffer (pH 7.4) containing 0.14 M NaCl and 0.05% Tween 20 by using a syringe.3. Fluorescence is detected by introducing an excess volume (ca. 100 μL) of 20 μM FDP in 50 mM Tris buffer (pH 9) with an incubation time of 60 min.
Fluorescein diphosphate tetraammonium is a fluorescent dye, it can be used as a fluorogenic substrate and generats fluorescein as a fluorescent product.
体外研究：
Fluorescein diphosphate tetraammonium can be used as a fluorogenic substrate, and obtains luorescein as a fluorescent product.Fluorescein diphosphate tetraammonium (50 μM; room temperature) can be used as a substrate in vitro phosphatase assays.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).ELISA with capillary immunosensor (single-step ELISA):1. Put various concentrations of antigen in Tris buffer 1 containing 1% BSA into capillary immunosensor.2. Immobilize capture antibody and enzyme-linked antibody via capillary action, incubates for a preset time, and subsequently washing with 1 mL of 50 mM Tris buffer (pH 7.4) containing 0.14 M NaCl and 0.05% Tween 20 by using a syringe.3. Fluorescence is detected by introducing an excess volume (ca. 100 μL) of 20 μM FDP in 50 mM Tris buffer (pH 9) with an incubation time of 60 min.
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