产品
编 号：F270229
分子式：C33H38F3N7O11
分子量：765.69
分子式：C33H38F3N7O11
分子量：765.69
产品类型
规格
价格
是否有货
1mg
询价
询价
5mg
询价
询价
10mg
询价
询价
结构图
CAS No: 210345-03-2
产品详情
生物活性：
Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).The assessment of the caspase activity by fluorogenic substrate assay:1. Preparation of cell lysis buffer: 1 % Nonidet P-40, NaCl 200 mM, Tris/HCl 20 mM, pH 7.4, Leupeptin 10 mg/L, Aprotinin (HY-P0017) (0.27 kU/L) and 100 mM PMSF (HY-B0496) .2. Incubate the lysis buffer (25 mg of total protein) with 50 mmol/L of AC-DEVD-AMC (HY-P1003) (caspase-3), AC-VEID-AFC (caspase-8) or AC-LEHD-AFC (caspase-9) in the buffer (HEPES 10 mM, pH 7.4, containing Mannitol 220 mM, Sucrose 68 mM, NaCl 2 mM, KH2PO4 2.5 mM, EGTA 0.5 mM, MgCl2 2 mM, Pyruvate 5 mM, PMSF 0.1 mM, and Dithiothreitol 1 mM).3. Assess the caspase activity by measuring fluorescent 7-amino-4-methylcoumarin released for 1 h at a 2-min intervals by a spectrofluorometer.
Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).The assessment of the caspase activity by fluorogenic substrate assay:1. Preparation of cell lysis buffer: 1 % Nonidet P-40, NaCl 200 mM, Tris/HCl 20 mM, pH 7.4, Leupeptin 10 mg/L, Aprotinin (HY-P0017) (0.27 kU/L) and 100 mM PMSF (HY-B0496) .2. Incubate the lysis buffer (25 mg of total protein) with 50 mmol/L of AC-DEVD-AMC (HY-P1003) (caspase-3), AC-VEID-AFC (caspase-8) or AC-LEHD-AFC (caspase-9) in the buffer (HEPES 10 mM, pH 7.4, containing Mannitol 220 mM, Sucrose 68 mM, NaCl 2 mM, KH2PO4 2.5 mM, EGTA 0.5 mM, MgCl2 2 mM, Pyruvate 5 mM, PMSF 0.1 mM, and Dithiothreitol 1 mM).3. Assess the caspase activity by measuring fluorescent 7-amino-4-methylcoumarin released for 1 h at a 2-min intervals by a spectrofluorometer.
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