产品
编 号：F229558
分子式：C28H40O4
分子量：440.61
分子式：C28H40O4
分子量：440.61
产品类型
规格
价格
是否有货
25mg
询价
询价
50mg
询价
询价
100mg
询价
询价
结构图
CAS No: 18323-58-5
产品详情
生物活性：
4-Methylumbelliferyl oleate is a fluorogenic substrate for acid and alkaline lipases. 4-Methylumbelliferyl oleate is cleaved by lipases, liberating 4-Methylumbelliferyl (Ex/Em=320/450 nm).
体外研究：
The inhibitory activity against pancreatic lipase was measured using 4-Methylumbelliferyl (4-MU) oleate as a substrate. The reaction mixture consisted of 50 μL 0.1 mM 4-MU oleate, 20 μL McIlvane buffer (0.1 M citrate-Na2 HPO4, pH 7.4), and 5 μL of sample solution. Porcine pancreatic lipase (25 μL) was added to the reaction mixture and the final volume was adjusted to 0.1 ml. After the mixture was incubated at 37℃ for 10 min, the amount of 4-MU released by the lipase was measured using a fluorescence multi-detection reader at an excitation wavelength of 320 nm and an emission wavelength of 450 nm.
4-Methylumbelliferyl oleate is a fluorogenic substrate for acid and alkaline lipases. 4-Methylumbelliferyl oleate is cleaved by lipases, liberating 4-Methylumbelliferyl (Ex/Em=320/450 nm).
体外研究：
The inhibitory activity against pancreatic lipase was measured using 4-Methylumbelliferyl (4-MU) oleate as a substrate. The reaction mixture consisted of 50 μL 0.1 mM 4-MU oleate, 20 μL McIlvane buffer (0.1 M citrate-Na2 HPO4, pH 7.4), and 5 μL of sample solution. Porcine pancreatic lipase (25 μL) was added to the reaction mixture and the final volume was adjusted to 0.1 ml. After the mixture was incubated at 37℃ for 10 min, the amount of 4-MU released by the lipase was measured using a fluorescence multi-detection reader at an excitation wavelength of 320 nm and an emission wavelength of 450 nm.
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