产品
编 号：F213609
分子式：C49H90N5O11P
分子量：956.24
分子式：C49H90N5O11P
分子量：956.24
产品类型
规格
价格
是否有货
1mg
询价
询价
5mg
询价
询价
10mg
询价
询价
结构图
CAS No: 178119-00-1
产品详情
生物活性：
NBD-PE is an effective lipid fluorescent probe (Excitation/Emission: 465/535 nm; Color: Green). NBD-PE offers a wide array of applications in membrane and cell biology.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Assay of phospholipid transfer activity:1. The reaction mixture containes 2 mg mitochondrial protein, 0.02 μmol of NBD-PE, 0.5 mmol of Tris-HCl, pH 7.4, and 0-200 μg of pH 5.l-supernatant protein.2. Samples are incubated at 37°C for 1 hour with gentle agitation.3. The reaction is terminated by placing samples in an ice bath for 10 min, followed by centrifugation at 15,000 g for 5 min.4. The supernatants are decanted, allowed to come to room temperature, and the absorbance (460 nm) and the relative fluorescence at 535 nm (excitation at 465 nm) are determined.5. Blanks containing only NBD-PE and exchange protein are run concomitantly to correct for possible interaction between NBD-PE and exchange protein.6. The percent exchange of NBD-PE is calculated from the differences between the absorbances or relative fluorescence in the supernatant fractions in the presence and absence of exchange protein.
NBD-PE is an effective lipid fluorescent probe (Excitation/Emission: 465/535 nm; Color: Green). NBD-PE offers a wide array of applications in membrane and cell biology.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Assay of phospholipid transfer activity:1. The reaction mixture containes 2 mg mitochondrial protein, 0.02 μmol of NBD-PE, 0.5 mmol of Tris-HCl, pH 7.4, and 0-200 μg of pH 5.l-supernatant protein.2. Samples are incubated at 37°C for 1 hour with gentle agitation.3. The reaction is terminated by placing samples in an ice bath for 10 min, followed by centrifugation at 15,000 g for 5 min.4. The supernatants are decanted, allowed to come to room temperature, and the absorbance (460 nm) and the relative fluorescence at 535 nm (excitation at 465 nm) are determined.5. Blanks containing only NBD-PE and exchange protein are run concomitantly to correct for possible interaction between NBD-PE and exchange protein.6. The percent exchange of NBD-PE is calculated from the differences between the absorbances or relative fluorescence in the supernatant fractions in the presence and absence of exchange protein.
产品资料
Copyright©2015-2024 沪ICP备2022026524号-1
沪公网安备