产品
编 号：F176726
分子式：C58H38N2O22
分子量：557.46
分子式：C58H38N2O22
分子量：557.46
产品类型
规格
价格
是否有货
5mg
询价
询价
10mg
询价
询价
结构图
CAS No: 150347-59-4
产品详情
生物活性：
CFDA-SE is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFDA-SE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus. CFDA-SE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488 nm, and can be used to detect the proliferation of cells.
体外研究：
CFDA-SE 工作液的配制1.1 储备液的配制取 1 mg CFDA-SE 溶于 0.1794 mL DMSO 中，得到 10 mM CFDA-SE。注：原液建议 -20℃ 或 -80℃ 避光保存，避免反复冻融。1.2 CFDA-SE 工作液的配制在无血清细胞培养中稀释原液注意：请根据实际情况调整 CFDA-SE 工作液的浓度。 细胞染色2.1 悬浮细胞：4℃，1000 g 离心 3-5 分钟，弃上清。PBS 洗涤 2 次，每次 5 分钟。贴壁细胞：弃去细胞培养基，加入胰蛋白酶解离细胞，制成单细胞悬液。4℃ 1000 g 离心 3-5 分钟，弃上清。PBS 洗涤 2 次，每次 5 分钟。2.2 加入 1 mL CFDA-SE 工作液，室温孵育 30 分钟。 2.3 4℃，400 g 离心 3-4 分钟2.4 PBS 洗涤 2 次，每次 5 分钟。2.5 用无血清细胞培养基或 PBS 重悬细胞，荧光显微镜或流式细胞仪检测。
CFDA-SE is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFDA-SE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus. CFDA-SE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488 nm, and can be used to detect the proliferation of cells.
体外研究：
CFDA-SE 工作液的配制1.1 储备液的配制取 1 mg CFDA-SE 溶于 0.1794 mL DMSO 中，得到 10 mM CFDA-SE。注：原液建议 -20℃ 或 -80℃ 避光保存，避免反复冻融。1.2 CFDA-SE 工作液的配制在无血清细胞培养中稀释原液注意：请根据实际情况调整 CFDA-SE 工作液的浓度。 细胞染色2.1 悬浮细胞：4℃，1000 g 离心 3-5 分钟，弃上清。PBS 洗涤 2 次，每次 5 分钟。贴壁细胞：弃去细胞培养基，加入胰蛋白酶解离细胞，制成单细胞悬液。4℃ 1000 g 离心 3-5 分钟，弃上清。PBS 洗涤 2 次，每次 5 分钟。2.2 加入 1 mL CFDA-SE 工作液，室温孵育 30 分钟。 2.3 4℃，400 g 离心 3-4 分钟2.4 PBS 洗涤 2 次，每次 5 分钟。2.5 用无血清细胞培养基或 PBS 重悬细胞，荧光显微镜或流式细胞仪检测。
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